FECAL MICROBIOTA OF PEOPLE WITH CROHN’S DISEASE DOES NOT CHANGE WITH ILEOCECAL RESECTION

نویسندگان

چکیده

Abstract INTRODUCTION Up to 80% of people with ileal Crohn’s disease (CD) will require an ileocecal resection (ICR). CD recurrence following surgery is common, although antibiotics can reduce short term rates. Following ICR, it unknown what happens the fecal microbiota and how relates recurrence. To further investigate this, we analyzed microbiomes patients (with without ICR) compared healthy controls. HYPOTHESIS Patients active inflammation have more dysbiosis their microbiomes, ileocolonic (ICR) improves this dysbiosis. METHODS We performed a cross sectional study samples (stratified by ICR status) DNA was isolated V4 hypervariable region 16S rRNA gene amplified sequenced using Illumina platform. Ecological metrics were applied characterize microbiomes. In our comparison, stratified based on most recent colonoscopy into groups: 1) history versus surgically naïve patients, 2) (in either ileum and/or colon), 3) none (colonic excluded). RESULTS Twenty-four (n=14, 58 % 38 controls provided samples. Global trends demonstrated that significantly reduced richness (or observed taxonomic units) (Figure 1 A, p<0.0001) Shannon diversity indices B, in Community principal component analysis Bray-Curtis pairwise dissimilarity shows distinct clustering between C). Richness index not different are those undergone 2 A B). C D) also significant vs E F). CONCLUSION characterized did impact could distinguish inflammation. paired therapies improve may affect

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ژورنال

عنوان ژورنال: Inflammatory Bowel Diseases

سال: 2022

ISSN: ['1078-0998', '1536-4844']

DOI: https://doi.org/10.1093/ibd/izac015.110